TABLE 2.
Primers designed for gene disruptiona
| Primer | Sequence (5′ → 3′) |
|---|---|
| H2 | (−1790)-ATACCCAAGGCTCGGCAAATAACATCTGCTAAATCTTCCATATATTCCT(−1741)CATTCCGGGGATCCGTCGACC |
| H5 | (−84)-CCCACAAGTTAAAGTGTCTGATATATCAGGCTCGGAGTGTTATAGAAAA(−135)AGTGTAGGCTGGAGCTGCTTC |
| H6 | (+14)-ATTTAGAAACTTGAGCCTGTTAACATAATCAAATTTTCTTTTGTTGTACATAAT(−40)GTGTAGGCTGGAGCTGCTTC |
| H10 | (−24)-CTTTTGTTGTACATAATATGTACCTCGTGAGCATATGTAGTGCTCGTTTC(−73)GTGTAGGCTGGAGCTGCTTC |
| H11 | (−34)-ACATAATATGTACCTCGTGAGCATATGTAGTGCTCGTTTCATCATGAAA(−83)TGTGTAGGCTGGAGCTGCTTC |
| H12 | (−44)-TACCTCGTGAGCATATGTAGTGCTCGTTTCATCATGAAATCCCACAAGT(−93)TGTGTAGGCTGGAGCTGCTTC |
| H13 | (−54)-GCATATGTAGTGCTCGTTTCATCATGAAATCCCACAAGTTAAAGTGTCT(−103)GGTGTAGGCTGGAGCTGCTTC |
| H13-1 | (−35)-CATAATATGTACCTCGTGAGCATATGTAGTGCTCGTTTCATCATGAAAT(−84)CGTGTAGGCTGGAGCTGCTTC |
| H13-2 | (−36)-ATAATATGTACCTCGTGAGCATATGTAGTGCTCGTTTCATCATGAAATC(−85)CGTGTAGGCTGGAGCTGCTTC |
| H13-3 | (−37)-TAATATGTACCTCGTGAGCATATGTAGTGCTCGTTTCATCATGAAATCCC(−86)GTGTAGGCTGGAGCTGCTTC |
a
Underlined sequences correspond to the flanking regions of the kanamycin resistance gene of pKD13 (7). Dotted sequences correspond to the sites of the icsB promoter which flank regions to be deleted. The numbers indicate the position relative to the icsB transcription start site at +1.