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. 2003 Sep;185(17):5314–5319. doi: 10.1128/JB.185.17.5314-5319.2003

FIG.1.

FIG.1.

Schematic map of the constructs used in this study. Constructs 1 (pDA-1, pInt-Veb, and pVeb), 2 (pDA-2, pInt-1999-Veb, pInt-1999*-Veb, p1999R-Veb, p1999-Veb, and p1999L-Veb), and 3 (pDA-3, pInt-1999-2000-Veb, and p1999R-2000R-Veb) were cloned from genomic DNAs of P. aeruginosa clinical isolates 14, 1, and JES, respectively. The blaVEB-1 gene was inserted in opposite orientation to Plac, thus removing any contribution of promoter Plac in β-lactamase expression. The stop codon resulting from a site-directed mutagenesis experiment is shown by an asterisk (construct pInt-1999*-Veb). Restriction sites that were used at each cloning step are underlined. The coding regions are shown as boxes, with an arrow indicating the orientation of their transcription. The IR of IS1999 and IS2000 are shown by filled and empty triangles, respectively. IRL and IRR of IS1999 are indicated for pDA-2. The broken arrows indicate the promoter Plac. Thin dashed lines indicate ligation in the multiple-cloning site of the shuttle vector pBBR1MCS.3.