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. 2003 Sep;185(17):5248–5262. doi: 10.1128/JB.185.17.5248-5262.2003

TABLE 2.

Efficiencies of plating

Bacterial strain Relevant characteristics Relative plating efficiency at 30°Ca of phage strainb:
φA1122 E6 E11 K18 T3 T7 T71.2YN28
A1122 Y. pestis reference strain 1 1.0 1.0 0.7 <10−8 7 × 10−7 NDc
IJ511 E. coli K-12 reference strain 5 × 10−5 1.0 1.0 1.0 1.0 1.0 1
IJ511/Flac Pif+ ND 5 × 10−7 2 × 10−7 4 × 10−7 1.0 <10−10 2 × 10−6
IJ531 IJ511 optA1 tonA2 ND 1.0 1.1 1.2 1.0 1.1 1.2
B E. coli B prototroph 4 × 10−8 0.8 1.3 0.8 0.9 0.8 0.8
BL2 E. coli B lig ND 1.5 0.8 1.0 1.0 0.9 0.8
BR3 E. coli B rpoC-E2258K ND 6 × 10−6 5 × 10−6 4 × 10−6 0.8 0.7 0.9
BL21 E. coli B GalhsdS 10−6 1.3 1.3 1.0 1.0 1.1 1.1
D2371-48 S. sonnei ND <10−10 <10−10 <10−10 0.7 3 × 10−7 ND
a

Expressed relative to A1122 for φA1122 and relative to IJ511 for φA1122 host range mutants and both T3 and T7.

b

The tail fiber protein of the host range mutant phage E6 contains residues 1 to 130 from T7, E11 contains T7 residues 118 to 125, and K18 is a spontaneous host range mutant containing the gp17-L523S change.

c

ND, not determined.