TABLE 2.
Efficiencies of plating
Bacterial strain | Relevant characteristics | Relative plating efficiency at 30°Ca of phage strainb:
|
||||||
---|---|---|---|---|---|---|---|---|
φA1122 | E6 | E11 | K18 | T3 | T7 | T71.2YN28 | ||
A1122 | Y. pestis reference strain | 1 | 1.0 | 1.0 | 0.7 | <10−8 | 7 × 10−7 | NDc |
IJ511 | E. coli K-12 reference strain | 5 × 10−5 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1 |
IJ511/Flac | Pif+ | ND | 5 × 10−7 | 2 × 10−7 | 4 × 10−7 | 1.0 | <10−10 | 2 × 10−6 |
IJ531 | IJ511 optA1 tonA2 | ND | 1.0 | 1.1 | 1.2 | 1.0 | 1.1 | 1.2 |
B | E. coli B prototroph | 4 × 10−8 | 0.8 | 1.3 | 0.8 | 0.9 | 0.8 | 0.8 |
BL2 | E. coli B lig | ND | 1.5 | 0.8 | 1.0 | 1.0 | 0.9 | 0.8 |
BR3 | E. coli B rpoC-E2258K | ND | 6 × 10−6 | 5 × 10−6 | 4 × 10−6 | 0.8 | 0.7 | 0.9 |
BL21 | E. coli B Gal−hsdS | 10−6 | 1.3 | 1.3 | 1.0 | 1.0 | 1.1 | 1.1 |
D2371-48 | S. sonnei | ND | <10−10 | <10−10 | <10−10 | 0.7 | 3 × 10−7 | ND |
Expressed relative to A1122 for φA1122 and relative to IJ511 for φA1122 host range mutants and both T3 and T7.
The tail fiber protein of the host range mutant phage E6 contains residues 1 to 130 from T7, E11 contains T7 residues 118 to 125, and K18 is a spontaneous host range mutant containing the gp17-L523S change.
ND, not determined.