TABLE 2.
Proteins that interact with GlnB and GlnK, identified by screening of an R. capsulatus DNA library for use in yeast two-hybrid studies
| Bait | No. of transformants of yeast strain CG1945 | Prey | No. of independent fusions | Full-length protein (amino acids) | Interacting region (amino acids) |
|---|---|---|---|---|---|
| GlnB | 4 × 106 | NtrB | 4 | 355 | 1-355 (3×), 112-355 (1×) |
| NifA2 | 1 | 582 | 9-544 | ||
| DraT | 1 | 270 | 5-270 | ||
| PcrA | 1 | 852 | 597-852 | ||
| GlnK | 2 × 106 | Era | 1 | 303 | 61-303 |