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α-Thujone and 7-hydroxy-α-thujone inhibit [3H]EBOB binding to mouse brain membranes. (A) IC50 determination for α-thujone and 7-hydroxy-α-thujone (mean ± SEM, n = 4). (B) Scatchard plots as average of duplicate measurements for [3H]EBOB alone (Kd 2.8 nM and Bmax 1,700 fmol/mg protein) and with α-thujone at 5 μM (Kd 4.1 and Bmax 1,700) and 25 μM (Kd 7.2 and Bmax 1,700).
