Figure 6.

Representative GC-MS- selected ion monitoring chromatograms for α-thujone and metabolites extracted from the mouse liver microsome-NADPH (P450) system and the brain of α-thujone-treated mice (50 mg/kg, i.p., 10 min after treatment). The major metabolite is 7-hydroxy-α-thujone. Four minor hydroxythujone metabolites are as follows: 1) 4-hydroxy-α; 3) 4-hydroxy-β; 2 and 4) others. Dehydro refers to 7,8-dehydro-α-thujone. Shaded peaks not derived from α-thujone are an endogenous substance (end) and the internal standard (IS). All thujone-derived metabolites fall within the chromatographic region shown.