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Confirmation of ectopic expression of ropB. The presence of mRNA for ropB (top), speB (middle), and the housekeeping gene glnQ (bottom) was determined for cultures at the time points indicated by nonquantitative RT-PCR. The strains evaluated were identical to those listed in Fig. 7. The results shown are representative of at least three different RT-PCR assays from three separate RNA isolations.
