TABLE 1.
Primers used in this study
| Name | Sequencea |
|---|---|
| speB-20primerext | CTGCATTTGCTTTTAACGCTAC |
| speB-600primerext | GTTTGTCAATGCTTTTATTGAC |
| ropB intergene | GGAAGACTGTCTGACAGAGCGG |
| ropB-stop-BamHI | CGCGGATCCCCTCAGGACAGTTTATGTTTAATGG |
| speB-3′-fwd | GGCGATTTCAGAATTGATGGCTG |
| speB-3′-rev | GCCACCAGTACCAAGAGCTGAAGGG |
| glnQ-5′ | GGAATTGATGCCAAATTCTACGAAGGTG |
| glnQ-3′ | GATAACACGGTTAGCTACTTGGCGAGC |
| pSPE-speB+ 180EcoRI | ATATCTTCTGGAATTCGTCAACCTGCTTTG |
| pSPE10-speB-1000BamHI | GAATGCCTAATGGATCCAACGGTTTCACAA |
| pSPE11-speB-800BamHI | GTCATATGTGGATCCTTTCTAATC |
| pSPE12-speB-700BamHI | GCAGCTATGAAGGATCCATAAGGTTAAAAGGAAG |
| pSPE13-speB-400BamHI | GGTGTTGTGGATCCTATCACGATC |
| pSPE14-speB-250BamHI | GATGATAAGTGGATCCACTCATAGCGTC |
| pSPE15-speB-20BamHI | GTACCGTTAAAAGGATCCGCAGTAG |
| pSPE17-700ropB-XhoI | CCTTATGGCCTCGAGATAGCTGC |
| pSPE17-inverse-250speB-XhoI | GATGAGAATACTCGAGTTGGGTTGTC |
| pROP-ropB + 100EcoRI | GTTTTTTGAATTCCTAATCTATTCAACGG |
| pROP10-speB360BamHI | CTTCTGCGCTTCGGGATCCTGCTTTG |
| pROP11ropB1600BamHI | CGATGTCGCGGATCCCATGAATGGTAATAG |
| pROP12-ropB1400BamHI | GCTAGGCAGACGGATCCGCAAGATCAGC |
| pROP13-ropB1200BamHI | CCATTAGTTGACTCGTAGGATCCTCCCTGG |
| pROP14-ropB1000BamHI | CCTTTTAACCTTATGGATCCATCATAGCTGC |
| pROP15-ropB900BamHI | CATATGACAGTGGATCCAACTATCGCATCTG |
| 5′-ropB-IFD | AAAACTGCAGGGAGGTCGTGACCAAAAAGGCGGC |
| 3′-ropB-IFD | AAAACTGCAGGGAGGTCGTGACCAAAAAGGCGGC |
| ropB-5′-rev-IFD | TCCCCCGGGTTCACCAATTTCCATATGTCAAGC |
| ropB-3′-fwd-IFD | TCCCCCGGGATTAAACATAAACTGTCCTGAGGC |
| rofA-5′-BglII | GGAAGATCTCATATATGAGCTTACCACG |
| rofA-3′-BamHI | CGCGGATCCAGTTCCTCACAATAATGG |
| ropB-5′-RBS-BamHI | CGCGGATCCGACATCAACTAGGAAGGC |
| ropB-stop-PstI | AAAACTGCAGCCTCAGGACAGTTTATGTTTAATGG |
| ropB-5′-SmaI | TCCCCCGGGATGGAAATTGGTGAAACCG |
| 5′-Upstream-pro | CAAGCCTTCCTAGTTGATGTC |
| 3′-Upstream-pro | GTCAATGCTTTTATTGACTTCTC |
| 5′-Downstream-pro | GAGAACGGTCCCATATCATC |
| speBpro-20rev | TCCCCCGGGCTGCATTTGCTTTTAACGGTAC |
a
Underlined sequences denote restriction site incorporated into primer.