TABLE 1.
ORFs of the SopEΦ phage
| ORF | Size (kb) | % Identity (blastx) | Presencea or function in P2 phage |
|---|---|---|---|
| y | 1.21 rev.b | 27, hypothetical protein YopC of Bacillus subtilis phage SPBc2 | — |
| int | 1.0 rev. | 53, int (phage 186) | Integrase |
| 1 | 0.63 rev. | 30, Cl protein (phage 186) | Immunity repression |
| 2 | 0.25 | 31, Apl (phage 186) | — |
| 3 | 0.51 | 89, hypothetical 18.1-kDa protein of Escherichia coli retron Ec67 | — |
| 4 | 0.2 | 82, Fil (phage 186) | — |
| 5 | 0.34 | 84, hypothetical 12.8-kDa protein of Escherichia coli retron Ec67 | — |
| 6 | 0.23 | 79, hypothetical protein 79 (phage 186); 31, Orf80 (phage P2) | — |
| 7 | 0.23 | 60, Orf80 (phage 186); 47, Orf82 (phage P2) | Origin of replication |
| 8 | 0.85 | 73, retron EC67 DNA adenine methylase | — |
| 9 | 2.2 | 35, A protein (phage 186); 31, gpA (phage P2) | Origin of replication |
| 10 | 0.19 | 90, hypothetical protein of Fels-2 prophage (serovar Typhimurium LT2) | — |
| 11 | 0.23 | 42, Tum95.5 (phage 186) | — |
| 12 | 1.1 | 29, unknown (Methanothermobacter, wolfeii prophage psiM100) | — |
| 13 | 0.38 | 39, unknown (Methanothermobacter, wolfeii prophage psiM100) | — |
| 14 | 0.58 | 22, hypothetical protein (Pyrococcus furiosus DSM 3638) | — |
| 15 | 0.98 | 34, hypothetical protein (Microbulbifer degradans 2-40) | — |
| 16 | 1.0 rev. | 64, gpQ (phage P2); 63, capsid portal protein (phage 186) | Capsid |
| 17 | 1.78 rev. | 63, gpP (phage P2); 63, terminase subUnit (phage 186) | Capsid |
| 18 | 0.83 | 55, gene V protein (phage 186); 44, gpO (phage P2) | Capsid |
| 19 | 1.0 | 55, gpN (phage P2); 55, major capsid protein (phage 186) | Capsid |
| 20 | 0.65 | 42, R protein (phage 186); 41, gpM (phage P2) | Capsid |
| 21 | 0.53 | 47, Q protein (phage 186); 40, gpL (phage P2) | Capsid |
| 22 | 0.2 | 59, gpX (phage P2) | Lysis |
| 23 | 0.21 | 100, phage-holin analog protein of Fels-2 prophage (serovar Typhimunum LT2) | — |
| 24 | 0.51 | 37, gp17 lysozyme (phage P1) | — |
| 25 | 0.43 | 32, LysB (phage P2) | Lysis |
| 26 | 0.5 | 49, N protein (phage 186); 54, gpR (phage P2) | Tail |
| 27 | 0.45 | 43, O protein (phage 186); 34, gpS (phage P2) | Tail |
| 28 | 0.58 | 40, gpV (phage P2) | Tail |
| 29 | 0.36 | 59, M protein (phage 186); 55, gpW (phage P2) | Tail |
| 30 | 0.9 | 60, P2 J (phage 186); 59, gpJ (phage P2) | Tail |
| 31 | 0.6 | 58, gpI (phage P2); 57, Orf38 (phage 186) | Tail |
| 32 | 1.6 | 42, gpH (phage P2) | Tail |
| 33 | 0.4 | 50, gpG (phage P2); 46, unnamed protein product (phage 186) | Tail |
| sopE | 0.72rev. | sopE (exchanged for a kanamycin cassette in SopEΦsopE::aphT) invasion-associated secreted protein | — |
| 34 | 0.2 | 69, DNA invertase from lambdoid prophage e14 | — |
| 35 | 1.18 | 67, gpFI (phage P2); 69, (phage 186) | Tail |
| 36 | 0.51 | 50, gpFII (phage P2); 55, (phage 186) | Tail |
| 37 | 0.3 | 43, gpE (phage P2) | Tail |
| 38 | 0.12 | 57, tail protein, probably produced by a translational frameshift from orf51 into orf52 (phage 186); 55, gpE + E′ (phage P2) | Tail |
| 39 | 2.8 | 26, G protein (phage 186) | Tail |
| 40 | 0.5 | 50, gpU (phage P2); 45, F protein (phage 186) | Tail |
| 41 | 1.1 | 50, gpD (phage P2); 50, D protein (phage 186) | Late gene expression |
| 42 | 0.26 | 63, B protein (phage 186); 60, Ogr (phage P2) | Late gene expression |
a
—, not present.
b
rev., reverse.