TABLE 3.
Effect of the ΔprfC mutation on the frequency of exconjugant formation
| Donor straina | Recipient strainb
|
Frequency of exconjugant formation (10−5)c | |
|---|---|---|---|
| recA | prfC | ||
| pGP704 | + | + | 8 |
| pattP | + | + | 25,000 |
| − | + | 43,000 | |
| − | − | 205 | |
| + | − | 78 | |
| SXT | + | + | 44 |
| + | − | 0.1 | |
a
The donor strain was E. coli SM10λpir containing either pGP704 or pattP or E. coli BW25113 prfC::SXT.
b
The recipient strains were the MG1655-derived strains CAG18439 (recA+) and KB1 (recA) and their ΔprfC derivatives. When pGP704 and pattP were used, the recipient strains expressed Int from pInt33, as shown in Fig. 1.
c
The frequency of exconjugant formation was calculated by determining the number of exconjugants per recipient and is the mean from two independent experiments. The mating experiments were carried out for 6 h at 37°C.