TABLE 2.
Normalized fluorescence intensities of RbsC mutants
| RbsC mutation | Normalized fluorescence intensitya | Avg intensity ± SD |
|---|---|---|
| T42C | 25.2 | |
| N45C | 27.0 | |
| N48C | 24.2 | |
| N146C | 15.5 | |
| N157C | 26.0 | |
| G167C | 17.6 | |
| E238C | 22.8 | |
| A245C | 17.0 | |
| G252C | 20.9 | |
| D256C | 19.5 | |
| S298C | 23.3 | |
| 21.7 ± 3.9 | ||
| E19C | 1.66 | |
| V55C | 1.14 | |
| V95C | 3.17 | |
| A97C | 2.80 | |
| V124C | 1.93 | |
| H192C | 1.10 | |
| A201C | 1.36 | |
| N205C | 2.65 | |
| V216C | 1.90 | |
| K272C | 2.36 | |
| L292C | 1.28 | |
| N318C | 2.38 | |
| 1.98 ± 0.69 | ||
| T69C | 0.156 | |
| I72C | 0.167 | |
| S78C | 0.319 | |
| T83C | 0.136 | |
| A109C | 0.206 | |
| I111C | 0.087 | |
| A120C | 0.108 | |
| None (wild type; C227) | 0.247 | |
| V262C | 0.079 | |
| I275C | 0.384 | |
| L279C | 0.070 | |
| V309C | 0.082 | |
| 0.17 ± 0.10 |
a
Band intensities were first quantified by using a program included with the PhosphorImager and then normalized by taking the ratio of the intensity to the intensity of an internal reference.