TABLE 3.
PilV dependency of the frequency of transfer to various recipient S. enterica serovar Typhimurium LT2 and E. coli K-12 waa strains carrying various waa+ genes from different origins
| Recipient strain | Transfer frequency ratioa for donor strain expressing:
|
Sensitivityb to phage:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PilVA | PilVA′ | PilVB | PilVB′ | PilVC | PilVC′ | PilVD′ | P22 | FO | Ffm | |
| SL3770 waa+ | 0.003 | 0.35 | 0.003 | 1 | 0.004 | 0.009 | 0.004 | + | + | − |
| SL3770/pRF009 | 0.014 | 0.89 | 0.009 | 1 | 0.67 | 0.20 | 0.014 | + | ± | − |
| SL3770/pRF005 | 0.006 | 0.57 | 0.003 | 1 | 0.007 | 0.30 | 0.006 | + | ± | ± |
| SL3770/pRF006 | >0.001 | 0.18 | >0.001 | 1 | >0.001 | 0.004 | >0.001 | + | + | − |
| SL3749 waaL | 0.010 | 0.78 | 0.009 | 1 | 0.010 | 0.022 | 0.009 | − | + | + |
| SL3749/pRF009 | 0.003 | 1 | 0.004 | 0.002 | 0.27 | 0.03 | 0.002 | + | − | + |
| SL3749/pRF005 | >0.001 | 1 | 0.001 | 0.007 | 0.001 | 0.05 | 0.001 | − | ± | + |
| SL3749/pRF006 | 0.002 | 1 | 0.002 | 0.30 | 0.003 | 0.009 | 0.002 | − | + | + |
| SL733 waaK | 0.003 | 0.85 | 0.001 | 0.064 | 0.002 | 1 | 0.002 | − | − | + |
| SL733/pRF009 | 0.001 | 1 | 0.003 | 0.001 | 0.30 | 0.06 | 0.001 | + | − | ± |
| SL733/pRF005 | 0.003 | 1 | 0.002 | 0.002 | 0.003 | 0.04 | 0.002 | − | − | + |
| SL733/pRF006 | 0.002 | 1 | 0.001 | 0.027 | 0.001 | 0.42 | 0.002 | − | − | + |
| SL3750 waaJ | 0.005 | 1 | 0.004 | 0.015 | 0.004 | 0.005 | 0.010 | − | − | + |
| SL3750/pRF009 | 0.001 | 1 | 0.001 | 0.001 | 0.001 | 0.001 | 0.002 | − | − | + |
| SL3750/pRF005 | 0.002 | 1 | 0.001 | 0.003 | 0.002 | 0.004 | 0.001 | − | − | + |
| SL3750/pRF006 | 0.002 | 1 | 0.002 | 0.006 | 0.005 | 0.007 | 0.004 | − | − | + |
| JM83 | 0.007 | 0.29 | 0.007 | 0.006 | 0.62 | 1 | 0.006 | − | − | + |
| JM83/pRF101 | 0.020 | 0.33 | 0.020 | 1 | 0.15 | 0.38 | 0.013 | − | + | + |
| JM83/pRF102 | 0.013 | 1 | 0.017 | 0.006 | 0.09 | 0.32 | 0.007 | − | − | + |
| CS2334 waaL | 0.016 | 0.68 | 0.021 | 0.019 | 0.015 | 1 | 0.016 | − | − | + |
| CS2334/pRF006 | 0.003 | 0.63 | 0.003 | >0.001 | 0.94 | 1 | 0.003 | − | − | + |
| CS2334/pRF102 | 0.001 | 1 | 0.002 | 0.001 | 0.002 | 0.52 | 0.001 | − | − | + |
| CS2529 waaU | 0.004 | 0.6 | 0.006 | 0.005 | 0.005 | 1 | 0.005 | − | − | + |
| CS2529/pRF005 | 0.039 | 0.48 | 0.030 | 0.017 | 0.38 | 1 | 0.017 | − | − | + |
| CS2529/pRF101 | 0.004 | 1 | 0.005 | 0.045 | 0.001 | 0.049 | >0.001 | − | + | + |
| CS2198 waaR | 0.007 | 1 | 0.007 | 0.007 | 0.007 | 0.007 | 0.007 | − | − | + |
| CS2198/pRF101 | 0.007 | 1 | 0.007 | 0.007 | 0.007 | 0.005 | 0.007 | − | − | + |
S. enterica serovar Typhimurium LT2 SL4213 and E. coli K-12 W3110 harboring pKK661 and one of seven pKK641 series plasmids were used as donor strains. S. enterica serovar Typhimurium LT2 and E. coli K-12 with and without various waa+ plasmids were used as recipient strains. Liquid matings were carried out at 37°C for 1.5 h. For each recipient strain, the ratio of the frequency of transfer of pKK661 from E. coli K-12 or serovar Typhimurium LT2 donor cells expressing various PilV adhesins to the highest transfer frequency (1.0) is estimated. High ratios are indicated by boldface type.
+, sensitive; ±, partially sensitive; −, resistant.