FIG. 3.

Immunoblots of isolated membrane fractions containing LPS lipid A (A), LPS O-antigen (B), Tgl (a lipoprotein required for assembly of TFP) (C), CglB (a lipoprotein required for adventurous gliding) (D), PilA (the structural pilin protein for TFP) (E), FibA (an extracellular matrix-associated zinc metalloprotease) (F), CsgA (a protein required for C-signaling) (G), and FrzCD (a methylated chemotaxis protein) (H). Total membrane (lane TM), IM peak I (lane I), HM peak II (lane II), HM peak IV (lane IV), OM peak V (lane V), and OM peak VI (lane VI) fractions were separated by SDS-PAGE for Western blotting. The numbers on the left indicate molecular masses of protein standards (in kilodaltons).