FIG. 4.

Two-dimensional SDS-polyacrylamide gel separated in the first dimension by pH 3.5 to 10 and in the second dimension on 10% polyacrylamide. (A) IM peak I; (B) HM peak IV; (C) OM peak V; (D) OM peak VI. Molecular masses (MW) (in kilodaltons) are indicated on the left. A series of internal, carbamylated carbonic anhydrase markers ranging from pH 5 to 7.3 are underlined on each gel. The rectangles indicate acidic proteins common to IM peak I and HM peak IV which did not resolve well. The stars in panel A indicate protein spots common to IM peak I and HM peak VI. The numbers in panels C and D indicate clusters of proteins that are differentially expressed in the two OM peaks.