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. Author manuscript; available in PMC: 2007 Mar 5.
Published in final edited form as: J Mol Cell Cardiol. 2006 Dec 8;42(2):422–431. doi: 10.1016/j.yjmcc.2006.10.017

Figure 4. Expression of dnPKCε prevents ET-stimulated redistribution of endogenous phosphorylated MARCKS to the myofilaments.

Figure 4

NRVM and ARVM were infected with Adv encoding either cytoβgal or dnPKCε (750 MOI, 24h), and then treated with ET (10nM, 10min). Cells were fixed and labeled with anti-pMARCKS pAb. Rhodamine-congugated rabbit anti-goat IgG (red) and FITC-conjugated phalloidin (green) were used for visualization by laser confocal microscopy (~1μm optical sections). Areas of apparent co-localization appear as yellow in the MERGED images.