Figure 3.

Lack of damage bypass by hPolθ; 0.5 nM hPolθ was incubated with 10 nM DNA substrate in the presence of a 100 μM concentration of each of the four dNTPs. Lane 1, undamaged DNA; lane 2, a cis-syn T–T dimer containing template; lane 3, a (6–4) T–T photoproduct containing template; and lane 4, an abasic site containing template. The arrow on the right indicates the position of the first T of the dimer, the first T of the (6–4) photoproduct, or the position of the abasic site in the template.