Table 1.

Steady-state kinetic values and misincorporation frequencies for hPolθ

Template residue	Incoming dNTP	Vmax, nM/min	Km, μM	Vmax/Km	finc
G	G	0.3  ± 0.02	284  ± 47	0.001	2.2 × 10−4
	A	0.7  ± 0.04	178  ± 37	0.004	8.9 × 10−4
	T	0.8  ± 0.04	638  ± 88	0.001	2.2 × 10−4
	C	1.8  ± 0.06	0.4  ± 0.05	4.5
A	G	0.3  ± 0.02	118  ± 41	0.003	3.0 × 10−3
	A	0.2  ± 0.01	350  ± 56	6 × 10−4	6.0 × 10−4
	T	2.2  ± 0.3	2.2  ± 0.8	1.0
	C	1.2  ± 0.1	1,420  ± 305	8 × 10−4	8.0 × 10−4
T	G	0.38  ± 0.05	117  ± 33	0.003	4.4 × 10−4
	A	1.9  ± 0.05	0.28  ± 0.09	6.8
	T	0.3  ± 0.02	213  ± 55	0.001	1.5 × 10−4
	C	1.0  ± 0.02	551  ± 33	0.002	2.9 × 10−4
C	G	1.9  ± 0.08	0.6  ± 0.1	3.2
	A	0.22  ± 0.01	251  ± 31	9 × 10−4	2.8 × 10−4
	T	0.07  ± 0.01	121  ± 41	6 × 10−4	1.9 × 10−4
	C	1.2  ± 0.4	346  ± 37	0.003	9.4 × 10−4