Figure 1.
Effect of Z-Phe-Ala-CHN2 on the morphology of T. brucei bloodstream forms in vivo. Mice that had been infected with the pleomorphic variant clone AnTat 1.1 were injected intraperitoneally with 250 mg kg-1 of Z-Phe-Ala-CHN2 or vehicle alone on days 3 and 4 p.i. On day 5 p.i., blood smears were prepared and stained with May-Grünwald's stain solution. Representative examples from Z-Phe-Ala-CHN2-treated mice (a) and control mice (b) are shown. Trypanosomes exposed to the inhibitor appeared stumpy-like with a blue-stained region (arrowhead) between the kinetoplast and the nucleus, a location that is consistent with that of the lysosome in bloodstream forms. k, kinetoplast; n, nucleus; LS, long-slender forms; SS, short-stumpy forms.