Table 3.
Distribution of functional classes and temporal gene expression clusters between predicted NF-κB target and non-target genes in INS-1 cells.
| Temporal cluster | P-value |
| cluster 1 | 0.006 |
| cluster 2 | 1 |
| cluster 3 | 0.66 |
| cluster 4 | 1 |
| cluster 5 | 0.36 |
| cluster 6 | 0.32 |
| cluster 7 | 0.87 |
| cluster 8 | 0.73 |
| cluster 9 | 1 |
| cluster 10 | 0.14 |
| cluster 11 | 0.64 |
| cluster 12 | 0.66 |
| cluster 13 | 0.87 |
| cluster 14 | 0.53 |
| cluster 15 | 1 |
| Functional class | P-value |
| Metabolism | 0.65 |
| Protein synthesis | 0.87 |
| Ionic channels | 0.48 |
| Hormones and growth factors | 1 |
| Cytokines, chemokines | 0.05 |
| Signal transduction | 0.94 |
| MHC related | 0.06 |
| Cell adhesion | 0.03 |
| Transcription factors | 0.45 |
| RNA synthesis | 1 |
| Cell cycle | 1 |
| Defense repair | 0.79 |
| Apoptosis ER stress | 1 |
| Miscellaneous | 0.81 |
The classifications involved 225 NO-independent and cytokine-regulated genes in INS-1E cells [6]. 32 of them were predicted as NF-κB targets by the present approach. P-value: statistical significance of the overlap between the genes belonging to the functional or temporal class and the set of predicted NF-κB target genes (hypergeometric probability distribution).