Figure 2.

ABH had different effects on growth of several cell lines. The cells were cultured in the presence (closed circle) or absence (open circle) of 0.1% ABH for the indicated times (days). (A) The viable cell number (top) was assessed by the trypan blue dye exclusion method, and the viability (bottom) was determined as the percentage of trypan blue-negative cells to total cells. Data are representative of two separate experiments with similar results. (B) The proliferation of adherent cell lines was expressed as OD after addition of tetrazolium salt. Data are representative of two separate experiments with similar results.