Fig. 2.

Amplifications of PCV2 DNA. The effect of the PCV2 forward and reverse primer concentrations on PCV2 amplification

The experiment was carried out with forward and reverse primer concentrations varying from 100 to 1000 nmol/L, The C _T value obtained from each set of amplification conditions was plotted against the concentrations of the forward or reverse primer. Left open arrow, when the reverse primer concentration was lowered from 1000 nmol/L to 100 nmol/L, the PCV2 C _T increased markedly by 1.8 cycles; the C _T only increased by 0.9 cycles if the reverse primer was adjusted to 200 nmol/L (right open arrow)