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. 2007 Mar;8(3):162–169. doi: 10.1631/jzus.2007.B0162

Table 1.

Variance analysis of C T values quantified by real-time PCR in serially diluted standard plasmid solutions

Concentration of standard plasmid (copies/ml) n Intra-assay variability
Inter-assay variability
CT
CV (%) CT
CV (%)
Mean SD Mean SD
109 10 14.2 0.08 0.56 14.5 0.18 1.24
107 10 20.8 0.06 0.29 20.7 0.06 0.29
105 10 26.7 0.22 0.82 26.8 0.16 0.59