Figure 1.
Murine and human squamous cell carcinoma cell lines were cultured in medium containing increasing doses of lactoferrin in doses ranging from 0 μM to 250 μM. Daily MTT assays were performed to assess cell growth. A. Human cell line UMSCC9 cultured with varying doses of lactoferrin in the medium. There is a dose-dependent decrease in cell number from day 3 and beyond (P<0.05). B. Murine cell lines O12 cultured with varying doses of lactoferrin in the medium. There is a dose-dependent decrease in cell number from day 3 and beyond (P<0.05). C. Murine cell line SCCVII cultured with varying doses of lactoferrin in the medium. D. O12 cells were cultured with varying doses of lactoferrin as well as molar ratios of iron to saturate the lactoferrin. The iron binding state of lactoferrin had no effect on tumor.