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Steady-state kinetic parameters for wild type and active site mutants of BsPFK
| Enzyme | Vmax, units/mg | K1/2, mM | Hill number nH |
|---|---|---|---|
| Wild type | 111 ± 1 | 0.034 ± 0.001 | 1.6 ± 0.1 |
| E161A | 99 ± 1 | 0.035 ± 0.001 | 1.2 ± 0.1 |
| R162A | 122 ± 2 | 1.10 ± 0.06 | 1.0 ± 0.1 |
| E161A/R162A | 108 ± 1 | 1.21 ± 0.05 | 1.1 ± 0.1 |
