Table 2.

Thermodynamic coupling parameters for wild type and all three active site mutants of BsPFK at 25°C

Enzyme	Kia○, mM	Kiy○, mM	Qay × 103	ΔGay, kcal/mol
Wild type	0.028  ± 0.002	0.040  ± 0.004	1.4  ± 0.1	3.89  ± 0.04
E161A	0.025  ± 0.002	0.106  ± 0.011	2.5  ± 0.7	3.55  ± 0.17
R162A	1.16   ± 0.06	1.01   ± 0.09	23    ± 4	2.23  ± 0.10
E161A/R162A	0.76   ± 0.06	0.166  ± 0.019	14   ± 1	2.53  ± 0.04

Parameters were determined from steady-state kinetics with MgATP = 3 mM.