Table 3.

Thermodynamic coupling parameters for wild type and the E161A/R162A mutant of BsPFK at 25°C

Enzyme	Kia○, μM	Kiy○, μM	Qay × 103	ΔGay, kcal/mol
Wild type	0.7  ± 0.2	17.9  ± 2.7	2.9  ± 0.5	+3.47  ± 0.10
E161A/R162A	14.4  ± 4.7	33.9  ± 6.2	23    ± 4	+2.24  ± 0.10

Parameters were determined from titrations of intrinsic fluorescence with [MgATP] = 0.