Table 4.

Summary of thermodynamic parameters at 25°C obtained from van't Hoff analyses of the coupling parameters for wild type and the E161A mutant of BsPFK

Enzyme	ΔGay, kcal/mol	ΔHay, kcal/mol	TΔSay, kcal/mol
Wild type	3.61  ± 0.81	−4.31  ± 0.81	−7.93  ± 0.01
E161A	3.58  ± 0.76	−12.77  ± 0.76	−16.35  ± 0.05