Fig. 3.

(a) Measurement of the transamidation activity of TG prepared from Caco2 cells and FHs 74 Int cells. gp-tTG was used as a standard. (b) and (c) Inhibition of the transamidation activity of Caco2-tTG, FHs 74 Int-tTG cells and gp-tTG by different gliadin peptides at 2 mM. (b) Native gliadin peptides: p56–88, p57–68 and p69–82 and p31–49. (c) Modified gliadin peptides p57–68 (E65) and p69–82 (E72). Results represent the mean of three experiments, each done in duplicate. (d) P57-68 mediated inhibition of the activity of tTG extracted from caco2 cells. (1) Control DMSO, (2) p57–68, and (3) reversal of inhibition on transferring the contents of (2) to wells without p57–68.