Fig. 6.

(a) In vitro culture of peripheral blood mononuclear cells (PBMC) with pamidronate; mean percentage ± s.e.m. Interferon (IFN)-γ positive lymphocyte subsets after in vitro culture of PBMC with pamidronate. Pamidronate directly induces secretion of IFN-γ from γδ T cells, but not other T cell subsets. GD = γδ T cells. (b) Contour plots from flow cytometry data demonstrating secretion of IFN-γ from γδ T cells in response to in-vitro stimulation with pamidronate. Upper panels (a and b) show gating on γδ T cells (unstimulated: a; pamidronate stimulation: b), whereas in lower panels gating is on CD4 T cells for comparison (unstimulated: c; pamidronate: d).