Table 1.

Effects of hydrogen peroxide (H₂O₂) associated or not with heat shock (HS) on necrosis of RCC cells.

	Control	Irradiation	H2O2	H2O2 + HS
% Necrotic cells	15 ± 17	15 ± 18	32 ± 12	34 ± 9
% Viable cells	68 ± 24	67 ± 24	60 ± 12	59 ± 10

Renal carcinoma cells (RCC) were gamma-radiated or treated with 1 mM H₂O₂ for 12 h combined or not with HS (44°C during 30 min). Necrotic cells were detected by annexin V–propidium iodide assay. Control was performed with untreated cells. n = 6 for control and irradiation and n = 3 for H₂O₂ and H₂O₂ + HS. Necrotic cells: A +, PI + viable cells: A-PI-.