Fig. 6.

Effect of resveratrol (RVT) and curcumin (CMN) on the CD4⁺CD25⁺ T regulatory (T_reg) cells. RVT and CMN were added in different concentrations (1, 5, 10, 20 μM) to concanavalin A (ConA)-stimulated lymphocytes and CD4⁺ and CD25⁺ were enumerated by phycoerythrin (PE)-labelled anti-CD4 and fluorescein isothiocyanate (FITC)-labelled anti-CD25 antibodies. The CD4⁺ CD25⁺ T_reg cells were analysed by flowcytometer and the data representing the bar diagrams are expressed as percentage positive CD4⁺ CD25⁺ cells calculated, taking into consideration the ConA-treated cells as 100%. The data shown are from duplicate determinations.