Fig. 3.

The effect of macrolides on the production of cytokines from naive T cells co-cultured with dendritic cells (DCs). DCs were pretreated with lipopolysaccharide (LPS) alone or LPS with azithromycin (AZM) or clarithromycin (CAM). Subsequently all DCs were co-cultured with naive T cells from mice spleen for 5 days. DCs alone or naive T cells alone were cultured in the same plates. The production of interleukin (IL)-10 (a), IL-2 (b), interferon (IFN)-γ (c) and IL-6 (d) by DCs were measured by enzyme-linked immunosorbent assay. Values are expressed as the mean ± standard error of five experiments. *P < 0·05 compared with control.