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. Author manuscript; available in PMC: 2007 Mar 6.
Published in final edited form as: J Steroid Biochem Mol Biol. 2006 Nov 7;102(1-5):128–138. doi: 10.1016/j.jsbmb.2006.09.003

Fig. 1.

Fig. 1

Growth inhibition of MCF-7/E2 tumors in response to different FUL formulations and dosing schedules. Thirty ovariectomized athymic nude mice were bitransplanted in the axillary mammary fat pads with MCF-7/E2 tumor pieces 1 mm3 in size. At the time of tumor implantation, the mice were separated into 6 treatment groups of 5 mice each, or 10 tumors per group. The treatment groups were Control (no treatment), 0.3 cm E2 silastic capsule implanted sc, and 4 groups of different formulations/dosing schedules of 10 mg total FUL per week plus the 0.3 cm E2 capsule sc. The 4 FUL formulations/dosing schedules corresponded to: 1) a 50 mg/ml suspension of FUL dissolved first in EtOH and then mixed with peanut oil, and administered 2 times per week as a 5 mg sc injection; 2) the clinically used Faslodex preparation consisting of a 50 mg/ml solution of FUL in EtOH and castor oil, and administered 5 times per week as a 2 mg sc injection; 3) a 50 mg/ml suspension of FUL dissolved first in DMSO and then mixed with peanut oil, and administered 2 times per week as a 5 mg sc injection; or 4) a 50 mg/ml solution of FUL in 100% DMSO, and administered daily 5 times per week as a 2 mg sc injection. Tumor growth was tracked by weekly measurements using Vernier calipers and calculating the tumor cross-sectional area according to the formula: (length/2 × width/2 × π ). The data shown represent the average tumor cross-sectional area (cm2) per group ± SE. The cross-sectional area of E2-treated tumors was statistically different from that of each of the four E2 + FUL groups (all P-values <0.0001). Also, the cross-sectional area of tumors in the E2 + 5 mg FUL (EtOH/Peanut Oil suspension given 2 days per week) was statistically different from those in the E2 + 5 mg FUL (DMSO/Peanut Oil suspension given 2 days per week) group (P = 0.0013). Likewise, the cross-sectional area of tumors in the E2 + 2 mg FUL (EtOH/Castor Oil solution given 5 days per week) group was statistically different from that of the E2 + 2 mg FUL (100% DMSO solution given 5 days per week) group (P = 0.0038).