Figure 5. MUC1 peptide vaccine significantly reduced burden of established MC38.MUC1 tumors in MUC1.Tg mice.
Tumors were established for 7 days by subcutaneous injection of MC38.MUC1 cells (1 x 106) into the flanks of MUC1.Tg mice. Tumor-bearing mice were immunized weekly with MUC1-based peptide vaccine in combination with pan-helper-peptide, CpG ODN and GM-CSF. Tumors were measured (cm) and final weight (gm) calculated as (L x W2)/2. A significant reduction in tumor growth was observed in the vaccine treatment compared to vehicle control or peptide alone (††p = 0.003). The adjuvants alone (CpG ODN + GM-CSF) also showed a significant reduction in tumor burden compared to vehicle or peptides only (†p = 0.01).