Figure 4.

Factors contributing to G1 arrest. (A-B) PCNA-GFP expression (green) in discs with tkv^a12 (A) and smo³ tkv^a12 (B) clones. Upregulation in the posterior part of the NPR only occurs in the smo³ tkv^a12 clones. (C-D) Eye disc with smo³ (C) and tkv^a12 (D) clones (absence of GFP in red), stained for Dap antibody in green. The loss of smo, but not tkv, causes the loss of Dap in the furrow (arrows). (E) Eye disc with ato¹ M⁺ clones (absence of green) showing no ectopic BrdU incorporation (red) in the NPR. (F) UAS-ato overexpression clones (marked by GFP in green) can inhibit CycB accumulation (red) close to the anterior part of the NPR (arrow in F').