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Experimental validation of HAVANA annotation. 'Known' and 'Novel_CDS' were submitted to 5' RACE, and 'Novel transcript' and 'Putative' loci were submitted to RT-PCR on all their exon junctions, followed by bi-directional RACE. Several steps of reannotation were performed during the process of experimental verification: the figure shows the update of the annotation between the first release in April 2005 and the release from October 2005.
