Figure 2.

P4 cell extracts were assayed for firefly and Renilla luciferase activities 24 h after transfection by cationic lipids. PGK/luc reporter constructs [(+)PPT, (+)PPTmut, (−)PPT] were introduced with a RL-expressing vector (TK/RL) in the presence or absence of various oligonucleotides, as indicated. Results are presented as normalized firefly/Renilla luminescence intensity ratios (relative luciferase activity). The use of either control oligonucleotide (15-I1 or 15-I2, indicated as 15-I) provided equivalent results. If two isolated transfections were performed to introduce plasmids first and oligonucleotides 3–7 h later, the same levels of modulation were observed [conditions indicated by (*)].