Table 1.

Kinetic parameters for antibody-catalyzed aldol and retro-aldol reactions

Substrate	Antibody	kcat, min−1*	Km, μM*	kcat/kun†	(kcat/Km)/kun‡
	40F12	0.0054	33	2.4  × 104	7.2  × 108
	42F1	0.0026	41	1.1  × 104	2.8  × 108
	38C2§	0.0067	17	2.9  × 104	1.7  × 109
	40F12	0.071	203	6.5  × 105	3.2  × 109
	42F1	0.048	271	4.3  × 105	1.6  × 109
	38C2	0.21	123	1.9  × 106	1.5  × 1010
	40F12	0.21	317	8.8  × 105	2.8  × 109
	42F1	0.14	266	5.8  × 105	2.2  × 109
	38C2	1.1	184	4.6  × 106	2.5  × 1010
	40F12	0.11	130	6.1  × 102	4.7  × 106
	33F12	0.21	317	1.2  × 103	3.8  × 106
	38C2	4.8	20	2.7  × 104	1.4  × 109
	40F12	0.11	130	1.1  × 105	8.5  × 108
	33F12	0.11	43	1.1  × 105	2.6  × 109
	38C2¶	1.0	14	1.0  × 106	7.1  × 1010

^*The kinetic data of k_cat and K_m (per antibody active site) were obtained in PBS at pH 7.4 by fitting experimental data to nonlinear regression analysis using grafit software. 

^†Aldol reactions with a unit (M). 

^‡Retro-aldol reactions with a unit (M⁻¹). 

^§Refs. 1 and 2. 

^¶Ref. 8.