Figure 3.

Comparison of EC mitogenic activity between avascular and vascular tumor nodules. Protein was extracted from nodules, dialyzed against PBS, and added (10 μg/well) to each well of capillary EC. Stimulation of EC proliferation by nodule extracts was expressed in comparison to maximal stimulation (100%) by basic fibroblast growth factor (1 ng/ml, 0.4 ng/well), which resulted in a 4.2-fold increase in proliferation. All assays were done in duplicates. This result is representative of three independent experiments.