Figure 8.

Suppression of angiogenesis and tumor growth by MMP-2 antisense in vivo. Vascular tumor nodules preincubated with 10 μM oligonucleotides inhibited the normal embryonic vasculature of the chick CAM (5/5) (A), in contrast to the control nodules that were pretreated with random oligonucleotides and that stimulated capillary growth (4/5) (B), and compared with untreated CAM control (C). Vascular tumor nodules that were preincubated with 10 μM MMP-2 antisense for 3 days and were implanted into animals (n = 6) resulted in 69% suppression of tumor growth in comparison to control nodules treated with random oligonucleotides (P < 0.05).