Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vivo transcription assay based on interference of the replication of pSV-no. plasmids by SP6 transcription. SP6 RNA polymerase is produced from the gene under the control of the lac promoter in plasmid pACSP6R. It recognizes an SP6 promoter variant on a pSV-no. plasmid and produces a transcript that contains a 3′ RNA I sequence. This RNA interacts with RNA II and reduces the pSV-no. copy number. Thus, the copy number depends on strength of the promoter variant.
