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Lagging strand synthesis is stimulated by ribonucleoside triphosphates. The reaction mixtures were prepared as described in Materials and Methods. ICP8 and the ribonucleoside triphosphates GTP, CTP, and UTP were omitted as indicated. (A) Products were analyzed by alkaline agarose gel electrophoresis. (B) The products were cleaved with MboI and were analyzed by PAGE.
