Figure 5.

3′ poly(A) suppresses the LSM1 requirement of 1a-induced RNA3 stabilization. (A) Northern blot analysis of 1a stimulation of globin-RE mRNA accumulation in WT and lsm1i yeast. Equal amounts of total yeast RNA were loaded in each lane. (B) Schematic of WT RNA3 and RNA3-poly(A), in which the tRNA-like 3′ end (3′ from a unique StuI site) was replaced by a poly(A) tail generated by the yeast ADH1 polyadenylation site. (C) Northern blot analysis of 1a stimulation of RNA3 and RNA3-poly(A) accumulation in WT and lsm1i yeast. RNA3 and RNA3-poly(A) were expressed from the GAL1 promoter and equal amounts of total yeast RNA were loaded in each lane. Averages and SEM from three or more experiments are shown for each sample.