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Cut-and-paste transposition. Recombination initiates by means of double-strand breaks at either end of the transposon (white rectangle) from donor DNA (black lines), exposing terminal 3′-OH groups. Once cut from donor DNA, this excised linear transposon intermediate is joined to the target DNA (gray lines). The 5′ transposon ends are flanked by short gaps; upon repair, a simple insertion product is generated.
