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Heterotrimer formation after over-expressing a glnK-glnB operon in a UTase negative strain. Shown is a Western blot of a native gel of cell extracts detected with monoclonal PII antibody 19G4 that cross-reacts with GlnK. Lanes: 1, PII encoded by pWVH102 (B+); 2, GlnK encoded by pWVH149 (K+); 3, expression of the glnK-glnB operon from pWVH180 (KB+). Two heterotrimers, (GlnK)2PII and GlnK(PII)2, in lane 3 migrate in between the homotrimeric PII and GlnK.
