Table 1.
E. coli strains and plasmids used in this study
| Strains/plasmids | Genotype/description | Ref. |
|---|---|---|
| Strain | ||
| YMC10 | endA1 thi-1 hsdR17 supE44 ΔlacU169 hutC (wt) | 16 |
| RB9040 | As YMC10, but glnD99∷Tn10 | 17 |
| RB9060 | As YMC10, but glnB2306 | 17 |
| WCH30 | As YMC10, but Ω(GmR)-ΔglnK1 | 18 |
| UNF3435 | As YMC10, but Ω(GmR)-ΔglnK1, ΔglnB2306 | 18 |
| Plasmid | ||
| pRJ1 | E. coli glnB expressed from λ PR-PL in pND707 | 9, 19 |
| pWVH102 | E. coli glnB expressed from PA1LacO-1 (22) in pBluescript II SK(+) (pBSK) | 20 |
| pWVH149 | E. coli glnK expressed from Plac in pBSK | 4 |
| pWVH180 | E. coli glnK glnB operon expressed from Plac in pBSK | This work |