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. 2000 Apr 4;97(8):3948–3953. doi: 10.1073/pnas.040574197

Figure 1.

Figure 1

Identification of the sequences required for the cholesterol response of human FAS promoter I. Details regarding construction of the plasmids, the transfection conditions, the culture media, and the calculation of RLUs are described in Materials and Methods. HepG2 cells were used in these experiments. The fold activation was derived by dividing the RLU values obtained with transfected cells grown in inducing medium with the RLU values obtained with transfected cells grown in repressing medium. LC9–2/SREmut contains a mutated SRE but otherwise is identical to LC9–2.