Figure 2.

Essentiality of the NF-Y-binding sequence for the cholesterol response of human FAS promoter I. The experimental conditions were as described in the legend to Fig. 1 and in Materials and Methods. LC9–2/NF-Ymut contains GGATC instead of the inverted NF-Y sequence ATTGG. LC9–2/NF-Yinst contains an additional 4 bp that were inserted between the NF-Y- and Sp-1-binding sites. In some TK reporter gene constructs, a plus (+) denotes that the FAS promoter insert is in the same orientation as in human FAS promoter I, and a minus (−) denotes that the FAS promoter insert is in the opposite orientation with respect to human FAS promoter I.