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Identification of two Sp-1-binding sites in human FAS promoter I. The experimental conditions were as described in the legends to Figs. 1 and 3 and in Materials and Methods. The Sp1mut constructs contained the mutated sequence GAATTCTTC instead of the wild-type sequence GGGCGGCGC. The Sp1inst constructs contained an additional 3 or 7 bp that had been inserted between the SREs and the downstream Sp-1-binding site.
