Bilayer recordings and I-V curves showing modulation of single-channel currents through the αHL-CH1 pore by βCD. (a), Current recording in the presence of 40 μM βCD added to the trans side of the bilayer. The chambers contained 10 mM K phosphate (pH 7.4), and 5 μM EDTA, with cis, 1000 mM KCl; trans, 100 mM KCl. (b), I–V curves for αHL-CH1 (□) and αHL-CH1·βCD (○). The data points represent mean values (±SD) from three different experiments for αHL-CH1 and two experiments for αHL-CH1·βCD. Arrows, Vr.